A Practical Guide to Frozen Section Technique

A Practical Guide to Frozen Section Technique

Language: English

Pages: 194

ISBN: 1441912339

Format: PDF / Kindle (mobi) / ePub


A Practical Guide to Frozen Section Technique offers an easy to learn approach to frozen section technique in the form of a highly illustrated handbook intended for onsite use in the laboratory. The book begins with a novel, clearly delineated, step by step approach to learning continuous motion brush technique. Emphasis is placed on recognizing and correcting artifacts during the preparation process. The book addresses all of the steps in the preparation of slides from cutting through cover-slipping. The author’s unique, original techniques for tissue embedding including face down embedding in steel well bars, frozen block cryoembedding and paper cryoembedding are detailed. Variables key to the quality of the preparation including block temperature, tissue properties and section thickness are detailed. The book also covers understanding the cryostat and basic maintenance and care.Sections covering techniques used in Mohs dermatologic surgery, and techniques used in basic animal and human research are discussed by noted experts in their field.

A Practical Guide to Frozen Section Technique will be of great value to pathologists, pathology residents in training and also experimental pathology researchers that rely upon this methodology to perform tissue analysis in research.

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compared with the disposables being used in the operating room. Some tissues such as tough collagenous tissues or calcified tissues can quickly dull the blade. The blade should be changed when the section quality begins to fall. I have occasionally found myself getting mediocre sections with a brand new blade and changed it to find the tissue cutting easily with the second blade. Changing the blade on every case is also an important safety measure. If you cut yourself on a new blade, you will

beware when prelabeling multiple slides or any container for that matter. If you pick up the wrong labeled slide, you can easily confuse two specimens. This can be a problem particularly in very busy settings handling many specimens at a time. Personally I like to label the slide once I have picked up the 6  Fixation, Staining and Coverslipping of Frozen Section Slides 127 tissue. It will prevent such errors but as a result occasionally I will pick up the tissue on the wrong side of the

tissue. 7  Microscopic Interpretation in the Frozen Section Setting 137 The more exiting “tough ones” are examples of things we have never seen before. This may be a rare tumor type or disease state, or more commonly an uncommon variation of something more familiar to us. So many tumors can have numerous variations that overlap with different entities. Just think of the variety and overlap of histologic patterns that can be seen in melanoma, mesothelioma, thymoma and renal cell carcinoma,

skill set required for histology on research animal tissue vs. clinical tissue. The author’s primary experience is in the area of brain research and so will discuss issues relating to frozen section in animal tissues in the context of brain tissues. 9.2 Sacrif ice Perfusion Animal researchers commonly sacrifice an animal from which internal tissue samples will be harvested by the following method: 1. Heavily anesthetize the animal 2. Open the chest cavity 3. Cut a knick in the wall of the

9.1). The project to develop and test this was supported by Small Business Innovative Research (SBIR) grant funds from National Institutes of Health/National Institute of General Medicine (NIH/NIGM). 176 C.W. Scouten Fig. 9.1  Perfusion Two™ apparatus for sacrifice perfusion. The bottle on the left serves as an air pressure tank, the next bottle is prewash solution, then fixative. The 4th bottle is optional for a postwash if desired Fig. 9.2  Perfusion Comparison. Three brains perfused by

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